Born and raised in Nima, Accra, John Kobina Louis felt the call to the priesthood early. After completing his education at St. Augustine's College in Cape Coast, he entered St. Peter's Regional Seminary in Pedu, where he studied ecclesiastical sciences for six years before being ordained a priest of the Archdiocese of Accra in 1992.

From his very first assignment as an assistant parish priest at Our Lady of Mercy Church in Tema — where he oversaw four parishes including St. Joseph the Worker — it was clear that Fr. Louis was not content to simply maintain; he was called to build. Over the next decade, he served as parish priest, financial administrator of the Archdiocese, director of the Pastoral Centre, and spiritual director to the Handmaids of the Divine Redeemer Sisters.

His intellectual passion led him to Rome, where he earned a Licentiate in Dogmatic Theology (STL) from the Pontifical Urbaniana University in 2004. He later completed a PhD in the Study of Religions at the University of Ghana in 2014, with a doctoral thesis on the reception of Vatican II ecclesiology in the Ghanaian Church.

As Cathedral Administrator of the Holy Spirit Cathedral in Accra for six years, and later as Academic Dean and Acting President of the Catholic Institute of Business and Technology (CIBT), Bishop Louis combined pastoral leadership with rigorous academic formation — shaping the next generation of Catholic thinkers in Ghana.

In 2019, he was appointed Vicar General of the Archdiocese of Accra. Then, on April 19, 2023, he was ordained an auxiliary bishop — a recognition of decades of faithful, tireless service to the Church in Ghana and beyond.